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Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma


Journal article


Pranvera Sadiku, A. Brenes, Rupert L. Mayer, Leila Reyes, P. Coelho, Gabi van Stralen, Ailiang Zhang, Manuel A. Sanchez-Garcia, E. Watts, I. Liaquat, Andrew J. M. Howden, Ikeoluwa Adekoya, A. Boldbaatar, A. MacRaild, Sarah Risbridger, Gillian M. Morrison, H. MacPherson, C. Bruce, Shonna Johnston, R. Grecian, F. Murphy, Steven M Pollard, Paul M Brennan, K. Mechtler, Sarah R. Walmsley
Nature Communications, vol. 17(1), 2025

DOI: 10.1038/s41467-025-67367-3

Semantic Scholar DOI PubMedCentral PubMed
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APA   Click to copy
Sadiku, P., Brenes, A., Mayer, R. L., Reyes, L., Coelho, P., van Stralen, G., … Walmsley, S. R. (2025). Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma. Nature Communications, 17(1). https://doi.org/ 10.1038/s41467-025-67367-3


Chicago/Turabian   Click to copy
Sadiku, Pranvera, A. Brenes, Rupert L. Mayer, Leila Reyes, P. Coelho, Gabi van Stralen, Ailiang Zhang, et al. “Single Cell Proteomic Analysis Defines Discrete Neutrophil Functional States in Human Glioblastoma.” Nature Communications 17, no. 1 (2025).


MLA   Click to copy
Sadiku, Pranvera, et al. “Single Cell Proteomic Analysis Defines Discrete Neutrophil Functional States in Human Glioblastoma.” Nature Communications, vol. 17, no. 1, 2025, doi: 10.1038/s41467-025-67367-3.


BibTeX   Click to copy

@article{pranvera2025a,
  title = {Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma},
  year = {2025},
  issue = {1},
  journal = {Nature Communications},
  volume = {17},
  doi = { 10.1038/s41467-025-67367-3},
  author = {Sadiku, Pranvera and Brenes, A. and Mayer, Rupert L. and Reyes, Leila and Coelho, P. and van Stralen, Gabi and Zhang, Ailiang and Sanchez-Garcia, Manuel A. and Watts, E. and Liaquat, I. and Howden, Andrew J. M. and Adekoya, Ikeoluwa and Boldbaatar, A. and MacRaild, A. and Risbridger, Sarah and Morrison, Gillian M. and MacPherson, H. and Bruce, C. and Johnston, Shonna and Grecian, R. and Murphy, F. and Pollard, Steven M and Brennan, Paul M and Mechtler, K. and Walmsley, Sarah R.}
}

Abstract

Neutrophils are vital innate immune cells shown to infiltrate glioblastomas, however we currently lack the molecular understanding of their functional states within the tumour niche. Given that neutrophils are known to display a prominent discordance between mRNA and protein abundance, we developed ultra-sensitive mini-bulk and single cell proteomic (SCP) workflows to study the heterogeneity of peripheral blood and tumour associated neutrophils (TAN) from patients with glioblastoma. Mini-bulk analysis enabled a deeper protein coverage of circulating immature, mature and TAN populations, defining signatures of maturity and demonstrating that TANs resemble mature circulating neutrophils. Analysis of the SCP data resulted in the detection of >1,100 proteins from a single TAN providing a detailed characterization of neutrophil subsets in glioblastoma. Our approach shows evidence of pathogenic and anti-tumorigenic clusters and discovers cell states invisible to scRNAseq, opening new opportunities to selectively target pro-tumoural neutrophil states.


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