Journal article
Nature Communications, vol. 17(1), 2025
APA
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Sadiku, P., Brenes, A., Mayer, R. L., Reyes, L., Coelho, P., van Stralen, G., … Walmsley, S. R. (2025). Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma. Nature Communications, 17(1). https://doi.org/ 10.1038/s41467-025-67367-3
Chicago/Turabian
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Sadiku, Pranvera, A. Brenes, Rupert L. Mayer, Leila Reyes, P. Coelho, Gabi van Stralen, Ailiang Zhang, et al. “Single Cell Proteomic Analysis Defines Discrete Neutrophil Functional States in Human Glioblastoma.” Nature Communications 17, no. 1 (2025).
MLA
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Sadiku, Pranvera, et al. “Single Cell Proteomic Analysis Defines Discrete Neutrophil Functional States in Human Glioblastoma.” Nature Communications, vol. 17, no. 1, 2025, doi: 10.1038/s41467-025-67367-3.
BibTeX Click to copy
@article{pranvera2025a,
title = {Single cell proteomic analysis defines discrete neutrophil functional states in human glioblastoma},
year = {2025},
issue = {1},
journal = {Nature Communications},
volume = {17},
doi = { 10.1038/s41467-025-67367-3},
author = {Sadiku, Pranvera and Brenes, A. and Mayer, Rupert L. and Reyes, Leila and Coelho, P. and van Stralen, Gabi and Zhang, Ailiang and Sanchez-Garcia, Manuel A. and Watts, E. and Liaquat, I. and Howden, Andrew J. M. and Adekoya, Ikeoluwa and Boldbaatar, A. and MacRaild, A. and Risbridger, Sarah and Morrison, Gillian M. and MacPherson, H. and Bruce, C. and Johnston, Shonna and Grecian, R. and Murphy, F. and Pollard, Steven M and Brennan, Paul M and Mechtler, K. and Walmsley, Sarah R.}
}
Neutrophils are vital innate immune cells shown to infiltrate glioblastomas, however we currently lack the molecular understanding of their functional states within the tumour niche. Given that neutrophils are known to display a prominent discordance between mRNA and protein abundance, we developed ultra-sensitive mini-bulk and single cell proteomic (SCP) workflows to study the heterogeneity of peripheral blood and tumour associated neutrophils (TAN) from patients with glioblastoma. Mini-bulk analysis enabled a deeper protein coverage of circulating immature, mature and TAN populations, defining signatures of maturity and demonstrating that TANs resemble mature circulating neutrophils. Analysis of the SCP data resulted in the detection of >1,100 proteins from a single TAN providing a detailed characterization of neutrophil subsets in glioblastoma. Our approach shows evidence of pathogenic and anti-tumorigenic clusters and discovers cell states invisible to scRNAseq, opening new opportunities to selectively target pro-tumoural neutrophil states.